|
KMID : 0613820100200030388
|
|
Journal of Life Science
2010 Volume.20 No. 3 p.388 ~ p.395
|
|
|
Inhibition of Cyclooxygenase-2 Activity and Prostaglandin E₂ Production through Down-regulation of NF-κB Activity by the Extracts of Fermented Beans
|
|
Lee Hye-Hyeon
Park Cheol
Kim Min-Jeong
Seo Min-Jeong
Choi Sung-Hyun
Jeong Yong-Kee
Choi Young-Hyun
|
|
|
Abstract
|
|
|
|
Cyclooxygenase (COX)-2 is generally known as an inducible enzyme, and it produces arachidonic acid to prostaglandin E2 (PGE₂), which has been demonstrated to play a critical role in inflammation. In the present study, we investigated the effects of the extracts of fermented beans including soybean (FS), black agabean (FBA) and yellow agabean (FYA), on the expression of COXs and production of PGE2 in U937 human promonocytic cells. Treatment of phorbol 12-myristate 13-acetate (PMA) significantly induced pro-inflammatory mediators such as COX-2 expression and PGE₂ production, whereas the levels of COX-1 remained unchanged. However, pre-treatment with FS, FBA and FYA significantly decreased PMA-induced COX-2 protein as well as mRNA, which is associated with inhibition of PGE₂ production. Moreover, FS, FBA and FYA markedly prevented the increase of nuclear translocation of nuclear factor kappa B (NF-κB) p65 by PMA. Our data indicate that the extracts of fermented beans exhibits anti-inflammatory properties by suppressing the transcription of pro-inflammatory cytokine genes through the NF-κB signaling pathway.
|
|
|
KEYWORD
|
|
|
Fermented beans, COX-2, PGE₂, NF-κB
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|